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Hemolytic activities of NP1-NP9 (500 nM each) in the presence of plasma proteins. NPs were pre-incubated with 55% of plasma in PBS following incubation with RBCs for 30 min and 24 hr at 37 °C. The mixture was centrifuged to detect the cell-free hemoglobin in the supernatant. % Hemolysis was calculated using water as positive control. Error bars represent standard deviation (n=3).
