Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Stem Cell Res. 2013 Feb 5;10(3):428–441. doi: 10.1016/j.scr.2013.01.010

Figure 4. P53 promotes ETO-induced PCD.

Figure 4

A. ESCs were treated with ETO or left nontreated and fixed and stained with p53 antibody. B. Western blot using ESC whole cell lysates that were treated with ETO or left nontreated and harvested at the indicated time points and probed with the indicated antibodies. Actin served as a loading control. C. Western blots displaying the levels of p53 and Beclin 1 proteins in ESCs treated with ETO or left nontreated and incubated with Spautin 1. D. Quantitation of protein levels in C using ImageJ software. Band intensities were normalized to respective actin controls. E. Flow cytometric death analysis of ESCs treated with ETO or left nontreated and incubated with either pifithrin α to prevent transactivation of p53 target genes or pifithrin μ to prevent any mitochondrial function of p53. F. Flow cytometric death analysis of p53 proficient (+/+) ESCs (WD44) or p53 deficient (−/−) ESCs.*p<0.05; #p<0.01