FIG. 1.

In vitro eVLP stimulation triggers mRNA expression for type I IFNs, proinflammatory cytokines, and ISGs in BMDCs. BMDCs generated by Flt3L were stimulated with 10 μg/mL of eVLP for indicated time period (h). Cont represents unstimulated cells at time 0. mRNA transcripts of indicated genes in (A) (Pan Ifnα, Ifnβ, Tnf-α, IL-6, IL-1β, and IL-12p40) and in (B) (Ifit1, Mx1, Oas1a, Stat1, Irf1, and Irf7) were measured by qRT-PCR, normalized with HPRT, and are expressed as fold induction. Data represent the mean of duplicate samples from 3 independent experiments±SEM. All data were assessed by Student's t-test and are denoted by ***P≤0.001, **P≤0.01 and *P≤0.05. BMDCs, bone marrow-derived DCs; eVLP, Ebola virus-like particles; Flt3L, Fms-like tyrosine kinase 3 ligand; IFNs, interferons; ISGs, IFN-stimulated genes; qRT-PCR, quantitative real-time PCR.