Table 1.
Cytokine Genes Do Not Require MYD88
| Fold induction (eVLP/control) | |||
|---|---|---|---|
| Genes analyzed | MyD88+/+ DCs | MyD88−/−DCs | Significance P value (≤0.05) |
| Pan IFNα | 2.8 | 4.4 | 0.04a |
| IFNβ | 5.6 | 8.4 | 0.03a |
| TNFα | 9.2 | 6.1 | 0.031a |
| Ifit1 | 4.0 | 8.6 | 0.002b |
| Mx1 | 1.8 | 2.2 | 0.06n.s. |
| Oas1a | 1.9 | 2.7 | 0.06n.s. |
| Stat1 | 3.3 | 4.4 | 0.04a |
| Irf1 | 4.2 | 5.8 | 0.01b |
| Irf7 | 6.9 | 8.0 | 0.04a |
a
P≤0.05; bP≤0.01; n.s., no significance.
BMDCs from MyD88+/+ or MyD88−/− mice were stimulated with eVLP, and mRNA expression of indicated cytokines was measured by qRT-PCR as in Fig. 3. Values represent the average of duplicate samples from 3 independent experiments±SEM.
BMDCs, bone marrow-derived DCs; DCs, dendritic cells; qRT-PCR, quantitative real-time PCR.