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TSMod and tension studies in Vinculin. (A) Structure of TSMod. Upper panel shows resting TSMod with high FRET, the lower panel shows TSMod with force loading and low FRET; (B) three constructs of vinculin. Vinculin tagged by Venus at C terminal, vinculin head domain tagged by TSMod as a force free control, and vinculin with TSMod inserted between head and tail domain for force measurements. TSMod, tension sensor module.
