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. Author manuscript; available in PMC: 2014 Feb 14.
Published in final edited form as: Biochem J. 2012 Apr 1;443(1):125–131. doi: 10.1042/BJ20111035

Figure 1. Effect of DMPC and PS concentration on PMCA.

Figure 1

Ca2+ -ATPase activity of purified recombinant PMCA4 and PMCA2 in the presence of 100 μM Ca2+ and as a function of DPMC (A) and PS (B) concentration. CaM activation was determined in the presence of 3 μM Ca2+ and 3 μM Ca2+ plus 100 nM CaM. PMCA4 was reconstituted with 30 μM DMPC or 30 μM DMPC/70 μM PS (C), and PMCA2 was reconstituted with 20 μM DMPC or 20 μM DMPC/50 μM PS (D).