Fig. 5.

The inhibition of PTPC opening and a ROS scavenger blocks oxidative stress-mediated translocation of mitochondrial glutaminase. Hela Cells were transfected with pEGFP-GA215 alone. Post-transfection 24 h, cells were pre-treated with 5 μM CsA or 10 mM NAC for 30 min, and then treated with 200 μM H₂O₂ for another 24 h. Cells were subjected to subcellular fractionation and western blotting analysis with anti-GFP antibody, and β-actin as a loading control