Fig. 6.

The inhibition of glutamate over-production by inhibiting HIV-1 replication and ROS generation decreases glutamate-mediated neurotoxicity. RCN were treated for 3 days with serum-free neurobasal medium or 30 % MCM collected from HIV-1-infected MDM with or without CsA or NAC. Cells were fixed and then subjected to ELISA with anti-MAP-2 antibody, and the absorbance was read at 450 nm. Data values represent SEM from three independent experiments (n=3). ^## denotes p<0.05 in comparison to control; ns denotes no significance between Con-MCM and serum-free neurobasal medium