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. 1978 May;75(5):2112–2116. doi: 10.1073/pnas.75.5.2112

Mapping of linear and circular forms of mouse mammary tumor virus DNA with restriction endonucleases: evidence for a large specific deletion occurring at high frequency during circularization.

P R Shank, J C Cohen, H E Varmus, K R Yamamoto, G M Ringold
PMCID: PMC392501  PMID: 209450

Abstract

Rat hepatoma cells infected with mouse mammary tumor virus contain multiple forms of unintegrated viral DNA when grown in the presence of glucocorticoids. Using the DNA transfer procedure of Southern, we have prepared restriction endonuclease fragment maps of these forms of viral DNA. The maps indicate that: (i) the major species of viral DNA is a linear molecule of 5.9 X 10(6) Mr located in the cytoplasm; (ii) the nuclei contain covalently closed circular viral DNA of two distinct sizes (5.1 X 10(6) and 5.9 X 10(6) Mr) in addition to linear molecules (5.9 X 10(6) Mr); (iii) the linear molecule has specific termini; (iv) there is extensive homology between regions at or near termini of the linear molecule; (v) the predominant form of circular DNA lacks 1.2 kilobase pairs present in both the larger circular molecule and the linear molecule; and (vi) the sequences deleted from the majority of the circular DNA molecules are located at the ends of the linear DNA that are joined during circularization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Canaani E., Duesberg P., Dina D. Cleavage map of linear mouse sarcoma virus DNA. Proc Natl Acad Sci U S A. 1977 Jan;74(1):29–33. doi: 10.1073/pnas.74.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Commerford S. L. Iodination of nucleic acids in vitro. Biochemistry. 1971 May 25;10(11):1993–2000. doi: 10.1021/bi00787a005. [DOI] [PubMed] [Google Scholar]
  3. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  4. Duesberg P. H., Cardiff R. D. Structural relationships between the RNA of mammary tumor virus and those of other RNA tumor viruses. Virology. 1968 Dec;36(4):696–700. doi: 10.1016/0042-6822(68)90206-7. [DOI] [PubMed] [Google Scholar]
  5. Friedrich R., Morris V. L., Goodman H. M., Bishop J. M., Varmus H. E. Differences between genomes of two strains of mouse mammary tumor virus as shown by partial RNA sequence analysis. Virology. 1976 Jul 15;72(2):330–340. doi: 10.1016/0042-6822(76)90162-8. [DOI] [PubMed] [Google Scholar]
  6. Fritsch E., Temin H. M. Formation and structure of infectious DNA of spleen necrosis virus. J Virol. 1977 Jan;21(1):119–130. doi: 10.1128/jvi.21.1.119-130.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Keller W., Wendel I. Stepwise relaxation of supercoiled SV40 DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):199–208. doi: 10.1101/sqb.1974.039.01.026. [DOI] [PubMed] [Google Scholar]
  9. Parks W. P., Scolnick E. M., Kozikowski E. H. Dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus. Science. 1974 Apr 12;184(4133):158–160. doi: 10.1126/science.184.4133.158. [DOI] [PubMed] [Google Scholar]
  10. Ringold G. M., Blair P. B., Bishop J. M., Varmus H. E. Nucleotide sequence homologies among mouse mammary tumor viruses. Virology. 1976 Apr;70(2):550–553. doi: 10.1016/0042-6822(76)90297-x. [DOI] [PubMed] [Google Scholar]
  11. Ringold G. M., Cardiff R. D., Varmus H. E., Yamamoto K. R. Infection of cultured rat hepatoma cells by mouse mammary tumor virus. Cell. 1977 Jan;10(1):11–18. doi: 10.1016/0092-8674(77)90134-9. [DOI] [PubMed] [Google Scholar]
  12. Ringold G. M., Yamamoto K. R., Shank P. R., Varmus H. E. Mouse mammary tumor virus DNA in infected rat cells: characterization of unintegrated forms. Cell. 1977 Jan;10(1):19–26. doi: 10.1016/0092-8674(77)90135-0. [DOI] [PubMed] [Google Scholar]
  13. Ringold G. M., Yamamoto K. R., Tomkins G. M., Bishop M., Varmus H. E. Dexamethasone-mediated induction of mouse mammary tumor virus RNA: a system for studying glucocorticoid action. Cell. 1975 Nov;6(3):299–305. doi: 10.1016/0092-8674(75)90181-6. [DOI] [PubMed] [Google Scholar]
  14. Shank P. R., Varmus H. E. Virus-specific DNA in the cytoplasm of avian sarcoma virus-infected cells is a precursor to covalently closed circular viral DNA in the nucleus. J Virol. 1978 Jan;25(1):104–104. doi: 10.1128/jvi.25.1.104-104.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smotkin D., Yoshimura F. K., Weinberg R. A. Infectious, linear, unintegrated DNA of Moloney murine leukemia virus. J Virol. 1976 Dec;20(3):621–626. doi: 10.1128/jvi.20.3.621-626.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Tal J., Kung H. J., Varmus H. E., Bishop J. M. Characterization of DNA complementary to nucleotide sequences adjacent to poly(A) at the 3'-terminus of the avian sarcoma virus genome. Virology. 1977 Jun 1;79(1):183–197. doi: 10.1016/0042-6822(77)90344-0. [DOI] [PubMed] [Google Scholar]
  18. Taylor J. M. An analysis of the role of tRNA species as primers for the transcription into DNA of RNA tumor virus genomes. Biochim Biophys Acta. 1977 Mar 21;473(1):57–71. doi: 10.1016/0304-419x(77)90007-5. [DOI] [PubMed] [Google Scholar]
  19. Taylor J. M., Illmensee R., Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. doi: 10.1016/0005-2787(76)90307-5. [DOI] [PubMed] [Google Scholar]
  20. Teramoto Y. A., Kufe D., Schlom J. Multiple antigenic determinants on the major surface glycoprotein of murine mammary tumor viruses. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3564–3568. doi: 10.1073/pnas.74.8.3564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vaidya A. B., Lasfargues E. Y., Heubel G., Lasfargues J. C., Moore D. H. Murine mammary tumor virus: characterization of infection of nonmurine cells. J Virol. 1976 Jun;18(3):911–917. doi: 10.1128/jvi.18.3.911-917.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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