Table 1.
GABA concentration–response curve parameters for WT and L9′S mutant containing αβδ receptors
| Subunit combination | GABA EC50 (μM) | nH | No. of mutants* |
|---|---|---|---|
| αβδ | 1.91 ± 0.47 | 1.06 ± 0.04 | 0 |
| αβδm | 0.46 ± 0.11 | 1.41 ± 0.12 | 1 |
| αβmδ | 0.11 ± 0.04 | 0.63 ± 0.06 | 2 |
| αmβδ | 0.12 ± 0.03 | 1.18 ± 0.19 | 2 |
| αmβδm | 0.08 ± 0.02 | 0.95 ± 0.07 | 3 |
*
Number of mutants within the pentamer assuming a 2α:2β:1δ stoichiometry. GABA dose–response curves were obtained from five to nine HEK cells expressing αβδ, αβδm, αβmδ, αmβδ or αmβδm receptors. The Hill equation was fitted to each data set, and the mean values for GABA potency (EC50) and the Hill slope (nH) are shown in the table as mean ± SEM.