Figure 2.

Micturition variables in C57Bl6/J mice (n = 8 per group) that were left untreated (intact; control groups) or received (p.o.) vehicle (1= CMC), ADX71441 (1, 3, 10 mg kg⁻¹), oxybutynin (100 mg kg⁻¹) in Experiment 1 (A–D) or vehicle (1= CMC), baclofen (1, 3, 6 mg kg⁻¹), oxybutynin (100 mg kg⁻¹) in Experiment 2 (E–H). All animals, except controls, were subsequently overhydrated with water before receiving the diuretic drug, furosemide and monitored for micturition (see text). The variables assessed included number of urinary events (A, E), latency to the first urinary event (s; B, F), total urinary volume (mL; C, G) and the average urinary volume (mL; D, H). Each point represents the observed mean (+ SEM) for 0–45 min of the 90-min period. ⁺P = 0.07, * P < 0.05, ** P < 0.01 compared with the corresponding Reference (Ref) group. ^#P < 0.05, ^## P < 0.01, ^### P < 0.001 compared with the corresponding control group.