Figure 3.

Detection of male cells in sections from a bone biopsy taken after postnatal transplantation. Fluorescence in situ hybridization analysis of 4-μm single sections for X and Y chromosomes using α-satellite probes for the centromeric regions. The bone sample was taken 9 months after postnatal transplantation at 8 years and 11 months of age from patient A. X chromosomes (red dots) and Y chromosomes (green dots) can be recognized in 4′,6-diamidino-2-phenylindole-stained cell nuclei (blue). In total, 4 Y-chromosome-positive cells were detected among 60,000 cells. The arrows indicate cells containing Y chromosomes. Original magnification, ×100.