Figure 5.

Adipogenic differentiation of ASCs isolated from pericardial and thymic remnant adipose tissue. (A): GPDH enzyme activity of induced donor-matched P2 ASCs at 7 days. Data are expressed as mean ± SD (n = 3, N = 6). ∗, Significantly different (p < .05). (B): Representative oil red O staining at 7 days post-induction. Scale bars represent 100 μm. Relationship between GPDH activity and donor BMI for ASCs derived from pericardial adipose tissue (C) and thymic remnant (D). (E): Representative endpoint RT-PCR analysis of adipogenic gene expression at 7 and 14 days (n = 3, N = 2) with GAPDH as the housekeeping gene. Abbreviations: BMI, body mass index; GPDH, glycerol-3-phosphate dehydrogenase; H, hypoxic (5% O₂/90% N₂/5% CO₂); N, normoxic (95% air/5% CO₂); PF, pericardial fat; TH, thymic remnant.