Figure 5.

Identification of mature hepatocyte populations for scale-up and drug toxicity testing. Pluripotent stem cells were differentiated to hepatocytes as previously described. (A, B): hESC hepatocytes were induced with 1 mM phenobarbital drug for 48 hours prior to analysis of CYP3A and CYP1A2 function. (C, D): iPSC hepatocytes were induced with 1 mM phenobarbital drug for 48 hours prior to analysis of CYP3A and CYP1A2 function. The activity of CYP3A and CYP1A2 was analyzed using Promega pGlo technology, and activity was measured on a luminometer (POLARstar Optima). Units of activity are expressed as relative light units (RLU)/milliliter (ml)/milligram (mg) of protein (RLU/ml/mg). Levels of significance were measured by Student's t test. A p value <.01 is denoted as ∗∗, n = 3. Abbreviations: ctrl, control; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; PB, phenobarbital.