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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Microbiol. 2014 Jan 7;91(4):679–693. doi: 10.1111/mmi.12485

Figure 4.

Figure 4

A predicted lysophosphatidic acid acyltransferase (LPAAT) is expressed in the blood stage and liver stage endoplasmic reticulum (ER) but not the apicoplast. The expression of the apicoplast-predicted LPAAT was assessed using the epitope-tagged PY01678myc parasite. Blood stage parasites expressed PY01678 that did not co-localize with the apicoplast marker acyl carrier protein (ACP) (A) but did co-localize with the ER marker binding immunoglobulin protein (BiP) (B). At 24 hours (C) and 48 hours (D) of liver stage development, strong PY01678 expression was seen and as for blood stage expression, expression co-localized with BiP. DNA was stained with 4',6-diamidino-2-phenylindole and differential interference contrast (DIC) images were captured. Scale bar: 10 µm.