Table 1.

List of transcripts exhibiting the strongest fold changes in transcript accumulation in response to ethanol production (expressed as log ₂ difference producer-control strain)

			(log 2 ) fold change
Annotation	Gene function	Day 4	Day 7	Day 11	Day 18
slr1295-int1 futA1	Iron transport system (ferric ions)	−1.10	−1.08	−0.34	−3.13
slr1295-int3 futA1	Iron transport system (ferric ions)	−1.06	−1.16	−0.38	−3.09
sll1198-as1 trmD (3′ extension of futA1 transcript)	tRNA (guanine-N1)-methyltransferase	−1.00	−1.01	−0.26	−2.98
sll0477-as2	Biopolymer transport ExbB-like protein	−0.43	−0.86	0.67	−2.77
sll1577 cpcB	Phycocyanin beta subunit	−1.97	−2.54	−2.16	−2.15
slr1192 adhA*	Alcohol dehydrogenase	1.95	1.85	1.49	1.82
NC-117	ncRNA ncl1390	0.10	0.25	0.83	1.93
slr1418-int2 pyrD	Dihydroorotate dehydrogenase	1.05	0.34	−0.56	2.03
NC-184	ncRNA ncl1740	0.22	0.12	−0.25	2.06
sll1809 rps8	Ribosomal protein S8	1.46	1.81	1.44	2.59
slr0254-as2	Hypothetical protein	0.45	0.31	−0.22	2.72
slr1897-5′UTR srrA**	Periplasmic sugar-binding protein of ABC transporter	2.28	1.90	2.58	3.29

The list is ordered according to fold change at day 18, with transcripts repressed in the upper part and those induced during ethanol production in the lower part. For comparison, values from all four time points are shown but only three mRNAs (cpcB, adhA and rps8) exhibited strong fold changes throughout the course of the experiment. int, gene internal transcript; as, detected transcript is in antisense orientation to mentioned gene. *Overexpressed in producer strain; **part of petJ promoter fragment used for overexpression of adhA.