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. 2014 Feb 14;9(2):e88311. doi: 10.1371/journal.pone.0088311

Figure 3. Production of DC in Flt3L and GM-CSF/IL-4 supplemented cultures of spleen.

Figure 3

(A) D0, D2, D4, D5, D6, D7 and adult spleen cells were cultured with Flt3L or GM-CSF/IL-4. Cell production was assessed using antibody staining and multicolor flow cytometry. Gates were set on bivariate plots using isotype control antibodies, and numbers in gates reflect % positive cells. Production of CD11b+CD11c− and CD11b+CD11c+ cells was calculated in terms of proportion of each subset amongst all CD11c+ and/or CD11b+ cells. Representation of CD11b+CD11c+MHC-II− (L-DC) and CD11b+CD11c+MHC-II+ (cDC-like) cells was calculated in terms of proportion amongst CD11b+CD11c+ cells. (A) Proportion of cells induced by Flt3L. (B) Proportion of cells induced by GM-CSF/IL-4.