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. 2014 Feb 14;9(2):e88311. doi: 10.1371/journal.pone.0088311

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© 2014 Petvises, O’Neill

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Spleen cell cultures were supplemented cultures with Flt3L and GM-CSF/IL-4 were analysed for production of CD11c⁺CD11b⁺ DC. These subsets were further distinguished by expression of MHC-II, F4/80, Ly6C, CD8, Ly6G and B220.