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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Biomaterials. 2014 Jan 4;35(9):2868–2877. doi: 10.1016/j.biomaterials.2013.12.030

Fig. 5. Reduced intracellular tension decreases the diffusion coefficients of TRPC6 and ganglioside GM1.

Fig. 5

(A) The fluorescence intensity image of a cell expressing TRPC6-YFP before photobleaching (left), and at 0 and 2 min after photobleaching (middle and right), respectively. Yellow broken cycle highlights the region of interest for photobleaching. (B) Left: the concentration map after photobleaching (0 min), computed by normalizing the fluorescence intensity with the images before photobleaching. Right: the concentration map overlaid with a triangular mesh generated in the mobile region. (C) The time course of fluorescence recovery in the photobleached area as marked in (A). (D) The estimated diffusion coefficients of TRPC6 and ganglioside GM1 in MEFs transfected with TRPC6-YFP or stained with FITC-CTxB. MEFs were also treated with or without 5 μM ML-7 for 1 hr before FRAP assay. “n” represents the cell number in each group. * indicates the significant difference between indicated groups (P < 0.05). Scale bar, 20 μm.