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. 2014 Feb 14;9(2):e88833. doi: 10.1371/journal.pone.0088833

Table 3. The changes of segmental concentration of γ-aminobutyric acid (GABA), glycine (Gly), glutamate (Glu) and aspartate (Asp) measured 5 weeks after complete spinal cord transection in the whole tissue homogenates of thoracic (Th) and lumbar (L) segments.

Neurotransmitter (NT) Spinal cordsegment NT concentration(µmol/g wet weight tissue) Percentage of intact (%)
Intact rats Spinal rats
GABA Th6–8 0.53±0.076 0.55±0.080 103
Th9–10 (lesion) 0.53±0.076 0.36±0.093 67*
L1–2 0.65±0.008 0.49±0.038 76
L3–6 0.74±0.045 0.79±0.114 108
Gly Th6–8 1.96±0.176 1.77±0.245 90
Th9–10 (lesion) 1.96±0.176 1.17±0.258 60***
L1–2 2.08±0.153 1.53±0.184 74*
L3–6 2.51±0.167 2.47±0.202 98
Glu Th6–8 2.01±0.168 1.97±0.258 98
Th9–10 (lesion) 2.01±0.168 1.60±0.343 80
L1–2 2.23±0.182 1.87±0.226 84
L3–6 2.58±0.244 2.68±0.169 104
Asp Th6–8 0.90±0.106 0.89±0.111 99
Th9–10 (lesion) 0.90±0.106 0.59±0.155 66*
L1–2 1.05±0.146 0.83±0.099 80
L3–6 1.26±0.059 1.42±0.129 113

All samples were measured simultaneously by means of HPLC and the measurements were carried out in quadruplicates. The data of four rats with complete spinal cord transection performed at thoracic segments (Th9–10) and four intact rats are presented. Data show means ± SD, Two way-ANOVA, Tukey post-hoc tests, *P<0.05, ***P<0.001.