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. 2014 Feb 14;9(2):e89145. doi: 10.1371/journal.pone.0089145

Figure 1. Quantitative real-time RT-PCR analysis of transcript level of GmRAV gene under SDs and LDs.

Figure 1

A, Tissue-specific expression of soybean GmRAV in SDs. Tissues tested are leaf (Tl), trifoliate leaf (TL), stem (St), root (RO), pod (PO), flower bud (FB), and immature seed (IS) (plants aged 21 d). B, Relative transcript levels of GmRAV mRNA in soybean leaves under SDs and LDs. Soybean leaves were harvested every 4 h for 48 h at 25-day-old under LDs and SDs. Open and closed boxes indicate days and nights. C, Time course-dependent expression in LDs. Soybean ‘Dong Nong 47’ plants were grown under LDs for 10 d and were transferred to LDs or SDs before sampling. Relative transcript levels were analyzed by qRT-PCR. D, Histochemical detection of GmRAV–GUS promoter activity in transgenic Arabidopsis seedlings. 4-day-old seedlings were grown on MS medium.