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. 2014 Feb 14;9(2):e89036. doi: 10.1371/journal.pone.0089036

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© 2014 Teo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Representative images of colorimetric changes to THP1-Blue cell culture supernatant, indicating SEAP activity of substrate medium. B. Inter-isolate variation in live Blastocystis in modulating effects on LPS stimulation of THP1-Blue monocytes. Detection of SEAP activity from cell culture supernatants of THP1-Blue monocytes incubated with various conditions. Live parasites incubated with THP1-Blue cells in numbers corresponding to ratio of 1 THP1-Blue cell to 10 parasites (1–10), or 1 THP1-Blue cell to 20 parasites (1–20). Live ST4-WR1 and not ST7-B significantly dampens LPS-mediated NF-κB activation in THP1-Blue monocytes. Live parasites of both isolates also significantly reduce background absorbance. C. AnnexinV-FITC PI viability assessment of THP1-Blue monocytes after exposure to live parasites. Slight but consistent decrease in viability in THP1-Blue monocytes after exposure to live parasites of both isolates. **, p<0.01, when analyzed against 1 µg/ml LPS positive control. ##, p<0.01, when analyzed against complete media negative control.