Figure 3. Ethidium-bromide–stained agarose gel of PCR reaction products from 12specimens through conventional method.

Lane M, 100-base-pair (bp) DNA ladder; lane 1, reference strain of Escherichia coli; lane2, reference strain of Pseudomonas aeruginosa; lane 3, reference strain of Staphylococcus aureus; lane 4 to lane 12, representative clinical strain of Escherichia coli 1-3, Staphylococcus aureus 1-3 and Pseudomonas aeruginosa 1-3; lane 13, negative control without DNA template.