Table 1. Sequence quality from the 12 samples in two methods.

Samples description	Improved Sanger sequencing/Conventional Sanger sequencing
	LOR	Low QV (base numbers)	PLQ (percentage of low QVs.%)	High QV (base numbers)	PHQ (percentage of high QVs.%)	Sample score
1. AS.44113 Escherichia coli	463/492	84/37	18.1/7.5	360/454	77.8/92.3	27/37
2. ATCC.27853 Pseudomonas aeruginosa	466/466	78/39	16.9/8.4	401/436	86.1/93.6	34/42
3. AS.26003 Staphylococcus aureus	501/469	51/39	10.2/8.3	433/437	86.4/93.2	38/34
4. Escherichia coli 1	490/479	56/47	11.4/9.8	432/450	88.2/93.9	35/37
5. Escherichia coli 2	469/491	85/53	18.1/10.8	401/449	85.5/91.4	36/38
6. Escherichia coli 3	476/476	96/36	20.2/7.56	394/460	82.8/96.6	36/42
7. Staphylococcus aureus 1	487/478	56/75	11.5/15.7	427/401	87.8/83.9	31/30
8. Staphylococcus aureus 2	488/486	68/47	13.9/9.67	418/441	85.7/90.7	29/32
9. Staphylococcus aureus 3	500/464	37/57	7.4/12.3	470/437	94.0/94.2	37/34
10. Pseudomonas aeruginosa 1	482/469	60/48	12.5/10.2	432/449	89.6/95.7	37/43
11. Pseudomonas aeruginosa 2	485/478	51/44	10.5/9.2	434/456	89.5/95.4	34/43
12. Pseudomonas aeruginosa 3	422/485	81/33	19.2/6.8	366/462	86.3/95.3	35/45
Population mean	477.4/477.8	66.9/46.3	14.0/9.7	414/444.3	86.7/93.0	34.1/38.1
Statistical text. P value	–	–	<0.05*	–	<0.05*	<0.05#

^*using Wilcoxon Matched-Pairs Signed-Ranks Test.

^#using Matched-Pairs t-text.