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. 2014 Feb 14;9(2):e89278. doi: 10.1371/journal.pone.0089278

Figure 2. Monitoring of vertical transmission of Beauveria bassiana strain EABb 04/01-Tip.

Figure 2

Seeds that showed a positive amplification for B. bassiana from Figure 1 were sown in a sterile soil. DNA was extracted from surface sterilised pieces of plant sampled at different stages of growth (rosette, early notching, end of notching, capsule formation, and seeds). M, ÓGene Ruler DNA ladder mix (Fermentas international Inc., Burlington, Ontario, Canada ); lanes: 1 to 24, plants grown from seed lots that showed positive amplification; lane 25, positive control (B. bassiana DNA); lane 26, negative control (water, 1st-PCR round); lane 27, negative control (water, 2nd-PCR round). (*) Area sampled. Lane numbers for the different growth stages do not necessarily refer to the same plant.