Fig. 1.

Delayed liver regeneration after PHx in simple fatty liver. a BrdU immunohistochemical staining (original magnification ×200), and BrdU labeling index. b The expression of PCNA protein detected by Western blotting. c The mRNA levels of cell-cycle related genes, cyclin D1, cyclin E2, Foxm1, cyclin A2, and cyclin B1 measured by real-time RT-PCR. d The activations of Akt and Erk1/2 evaluated by Western blotting (black squares Control, white squares HFD, n = 5–8; mean ± SE, *p < 0.05, **p < 0.01, and ***p < 0.005 HFD mice vs. CD mice in each time point by ANOVA and Wilcoxon test)