Fig. 4.

Enhanced ER stress was observed in simple fatty liver during liver regeneration. a The mRNA levels of ER stress-related genes, GRP78, IRE1α, ATF6, PERK, sXBP-1, and CHOP measured by real-time RT-PCR. b The expression of GRP78 protein detected by Western blotting and immunohistochemical staining (original magnification ×100). c The expression of sXBP-1 protein detected by Western blotting and immunohistochemical staining (original magnification ×200) (black squares Control, white squares HFD, n = 5–8; mean ± SE, *p < 0.05 and **p < 0.01 HFD mice vs. CD mice in each time point by ANOVA and Wilcoxon test)