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. 2014 Jan 3;3(2):129–137. doi: 10.1242/bio.20146841

Fig. 5. The stable expression of LOXL2 mutants in MDCK cells induces EMT.

Fig. 5.

Characterization of MDCK transfectants obtained after stable expression of wild-type LOXL2, and the LOXLi mutants ΔLOXL2 or H626/628Q. (A) Western blot analyses were performed on whole cell extracts for the expression of ectopically expressed LOXL2 variants, E-cadherin, N-cadherin, fibronectin and vimentin. α-tubulin was used as a loading control. (B) Confocal immunofluorescence images for ectopically expressed LOXL2 variants (red), E-cadherin/ZO-1/vimentin (green) and F-actin (magenta) in the indicated clones. Phase contrast images of the indicated cells are shown at the left panels. Scale bar: 50 µm.