Fig. 4. Feminization of ppk23-expressing cells leads to significant shifts in the chemosensory transcriptome in male appendages.

(A) Real-time quantitative RT-PCR analyses of chemosensory genes that have been previously implicated in pheromonal sensing. Analyses were of total RNA extracted from male appendages from feminized flies (w¹¹¹⁸;ppk23-GAL4/UAS-tra^F) and wild-type controls (w¹¹¹⁸/UAS-tra^F). (B) Real-time quantitative RT-PCR analyses of members of the Cytochrome P450 family, subfamily 6. The expression of Cyp6d2 is shown separately since this gene was regulated in the opposite direction relative to all other Cyp6 genes (C). (D) PCR analyses of sex-specific fru transcripts in appendages. fru^M, male-specific; fru^F, female-specific; fru^C, common exons. M  =  male, F  =  female, +/+ = w¹¹¹⁸. *p<0.05; **p<0.01.