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. 2013 Nov 22;20(3):260–270. doi: 10.1093/molehr/gat087

Figure 7.

Figure 7

(A) End-point RT–PCR for Cx43 and GAPDH mRNA in ESC from four control (Ca–d) and four endometriosis (Ea–d) subjects, incubated in the absence (−) or presence (+) of E/P/c for 7 days. The expected amplicons of 292 and 185 bp, respectively, were noted. 100-bp molecular size markers in the flanking lanes. (B) Steady-state Cx43 mRNA levels were quantified by qRT–PCR and normalized to GAPDH in ESC from a control subject (C) and from an endometriosis case (E). Accumulation over a 7-day time course of E/P/c was compared. Inset shows amplification profiles and melting curves of qRT–PCR. (C) qRT–PCR in nine control and nine endometriosis ESC samples was performed to measure steady-state Cx43 (stippled histograms), Cx32 (dark histograms) or Cx26 (striped histograms) mRNA concentrations under basal conditions (left panel) and after 24 h of E/P/c treatment (right panel).