Skip to main content
. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: J Comp Neurol. 2012 Apr 1;520(5):1047–1061. doi: 10.1002/cne.22769

Figure 1.

Figure 1

Preprogalanin (PPGal) mRNA in the retrotrapezoid nucleus. A1: PPGal mRNA-containing neurons (brightfield illumination) with mRNA revealed by nonradioactive digoxigenin immunohistochemistry using alkaline phosphatase and nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate as substrate (see Stornetta et al., 2009 for details). A2: Same field photographed under fluorescent light to reveal the Phox2b-immnoreactive (ir) nuclei. Every PPGal-mRNA-positive neuron contains a Phox2b-ir nucleus. B: Photomontage illustrating the isolation of the cluster of PPGal-mRNA-positive retrotrapezoid nucleus (RTN) neurons. Scale bar in A1 = 100 μm for A1, A2; 330 μm for B.