Figure 3.

Overview of the mutations introduced into the CfMV genome. (A) Mutations in the arginine rich region of CP and the corresponding mutations in RdRp. The RdRp is translated in-1 reading frame in relation to CP. Wild type (wt-RdRp and wt-CP) and mutated (R5X-RdRp, R5X-CP, R3L-RdRp and R3L-CP) amino acid sequences of the changed regions are indicated in single letter code. CfMV genome: Pro, protease domain, VPg, VPg domain. (B) Representation of CfMV mutant noCP, in which the initiation codon of CP is mutated and an additional stop codon, indicated with an asterisk, is introduced into the CP reading frame after RdRp sequence.