Figure 5.

Detection of CfMV and mutant noCP in biolistically inoculated wheat and barley plants. The host plant is indicated above the panels. RT-PCR – Reverse transcription PCR analysis of total RNA extracted from plants inoculated with CfMV (WT) and CfMV CP deletion mutant (noCP). Samples from a non-inoculated plant (n.i.) were used as negative control. Samples were collected from the inoculated leaves (Inoculated) at 14 dpi and from upper leaves (Systemic) at 21 dpi. Primers amplifying the region of RdRp and CP genes were used. W.blot–Western blot analysis of plant total protein extracts with polyclonal anti CfMV CP antibody (CP-ab). Ribulose-1,5-bisphosphate carboxylase oxygenase large subunit (RuBP-L) was visualised with Ponceau S stain as loading control.