Table 1.
Microarray data of genes involved in miRNA and siRNA biogenesis in WT, pap2 , OE7 and OE21
| Gene | WT | pap2 | OE7 | OE21 | OE7 FC | OE21 FC | P OE7 | P OE21 | Primary gene symbol |
|---|---|---|---|---|---|---|---|---|---|
| AT1G48410 |
9057 |
9424 |
10052 |
10717 |
111% |
119% |
0.322 |
0.057 |
Argonaute 1 (AGO1) |
| AT2G27880 |
225 |
218 |
415 |
354 |
183% |
154% |
0.037 |
0.048 |
Argonaute 5 (AGO5)* |
| AT1G69440 |
1073 |
853 |
2149 |
2357 |
200% |
219% |
0.002 |
0.000 |
Argonaute 7 (AGO7)* |
| AT5G44200 |
3599 |
3013 |
4053 |
4057 |
113% |
113% |
0.234 |
0.299 |
CPB20 |
| AT2G13540 |
6824 |
6887 |
6310 |
6274 |
92% |
92% |
0.415 |
0.440 |
CPB80 |
| AT1G01040 |
1543 |
1308 |
2319 |
2501 |
150% |
162% |
0.016 |
0.006 |
Dicer-like 1 (DCL1)* |
| AT3G43920 |
2701 |
2633 |
1984 |
2042 |
74% |
75% |
0.046 |
0.029 |
Dicer-like 3 (DCL3)* |
| AT4G20910 |
1308 |
1257 |
1139 |
1403 |
87% |
107% |
0.364 |
0.619 |
HEN1 |
| AT1G09700 |
2576 |
2610 |
3368 |
3881 |
130% |
150% |
0.049 |
0.005 |
Hyponastic leaves 1 (HYL1)* |
| AT1G14790 |
1673 |
1576 |
2528 |
2874 |
149% |
170% |
0.052 |
0.009 |
RNA-dependent rna polymerase 1 (RDR1)* |
| AT5G23570 |
2079 |
2333 |
5530 |
6674 |
265% |
322% |
0.011 |
0.000 |
Suppressor of gene silencing 3 (SGS3)* |
| AT3G50100 | 1588 | 1368 | 446 | 647 | 28% | 41% | 0.004 | 0.000 | Small RNA degrading nuclease 1 (SDN1)* |
*Significantly (p < 0.05) changed in both OE lines. FC: Fold change.
The following genes were not significantly (p > 0.05) different between the WT and OE lines:
AGO2, AGO4, AGO6, DCL2, DCL4, HEN 1, HEN2, HEN4, HASTY, NRPD1A, RDR2, RDR6 (SGS2), SDE3 and SDE5.