Fig. 4.

LC-MS profiles of enoxaparin (a), RO-enoxaparin (c) and the corresponding expanded chromatograms (b, d)

LC conditions: column C18 100 x 2.1 mm,.2.6 μm; eluents A and B – 10 mM DBA and 10 mM CH₃COOH in H₂O-CH₃CN = 9:1 (v/v) and CH₃CN, respectively; gradient: 0 min – 0%B, 5 min – 0%B, 130 min – 35%B, 140 min – 70%B, 145 min – 70%B, 148 min – 0%B, 170 min – 0%B; flow rate – 0.25 ml/min