Table 1.

Gradient used and the LMWH quantity loaded for the LC-MS profiling of the LMWHs and their RO-derivatives

Enoxaparin and RO-enoxaparin		Tinzaparin and RO-tinzaparin		Dalteparin and RO-dalteparin
Multistep gradient
Time (min)	% Eluent B	Time (min)	% Eluent B	Time (min)	% Eluent B
				0	10
0	0	0	5	4	10
5	0	5	5	15	18
130	35	125	35	30	18
140	70	130	70	115	35
145	70	135	70	133	70
148	0	138	5	138	70
170	0	160	5	140	10
				160	10
Sample quantity loaded, μg
15	15	30	30	30	30
Corresponding Figure
Fig. 4 and ESM Fig. S5		ESM Fig. S6		ESM Fig. S7

LC conditions: column C18 100 x 2.1 mm, 2.6 μm; eluents A and B – 10 mM DBA and 10 mM CH₃COOH in H₂O-CH₃CN = 9:1 (v/v) and CH₃CN, respectively; flow rate – 0.25 ml/min