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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Platelets. 2008 May;19(3):182–191. doi: 10.1080/09537100701493794

Figure 2.

Figure 2

Reaction kinetics of rFVIIa disassociation from RL platelets. RL platelets and rFVIIa were incubated in the presence of calcium as described in Figure 1 for an hour. As detailed in the Materials and methods section, the RL platelets were separated from unbound rFVIIa, then suspended in plasma or citrated saline to initiate the disassociation reaction. Portions of the reaction mixture were withdrawn and examined with Western analysis to quantify the amount of rFVIIa that remained associated with the RL platelets. Inset: Representative lanes from the Western analysis of rFVIIa~RL platelet conjugates one minute and two hours into the disassociation process.