Table 1.
Preinfusion experiment. Effect of rcFVIIa and rhFVIIa spiked in canine haemophilia A blood assessed by kaolin-activated thromboelastography.
| Phenotype | Dose (μg mL−1) | Compound | R (min) | K (min) | α (°) | MA (mm) |
|---|---|---|---|---|---|---|
| Haemophilia A | – | Buffer | >60 | – | – | – |
| Haemophilia A | 2.5 | rcFVIIa | 3.8 ± 0.3 | 1.2 ± 0.1 | 73 ± 0.9 | 81 ± 1.1 |
| 1.25 | rcFVIIa | 6.8 ± 1.1 | 2.8 ± 0.4 | 60 ± 2.4 | 79 ± 1.4 | |
| 0.5 | rcFVIIa | 16 ± 1.7 | 5.3 ± 0.6 | 40 ± 4.3 | 78 ± 3.9 | |
| Haemophilia A | 2.5 | rhFVIIa | 31 ± 3.9 | 10 ± 4.9 | 26 ± 14 | 61 ± 15 |
| 1.25 | rhFVIIa | 39 ± 6.1 | 19 ± 12 | 18 ± 13 | 48 ± 19 | |
| 0.5 | rhFVIIa | 56 ± 17 | – | – | – | |
| Normal | – | Buffer | 5.3 ± 1.0 | 2.6 ± 1.2 | 56 ± 10 | 65 ± 5.5 |
| Normal | 2.5 | rcFVIIa | 2.2 ± 0.5 | 1.1 ± 0.2 | 73 ± 3.2 | 69 ± 3.4 |
| 1.25 | rcFVIIa | 2.9 ± 0.7 | 1.2 ± 0.2 | 71 ± 2.8 | 65 ± 6.0 | |
| 0.5 | rcFVIIa | 3.6 ± 0.7 | 1.6 ± 0.2 | 68 ± 3.1 | 68 ± 4.5 | |
| Normal | 2.5 | rhFVIIa | 6.1 ± 0.1 | 2.2 ± 0.3 | 61 ± 3.7 | 61 ± 4.4 |
| 1.25 | rhFVIIa | 5.8 ± 0.1 | 2.0 ± 0.3 | 61 ± 3.7 | 61 ± 4.4 | |
| 0.5 | rhFVIIa | 5.5 ± 0.6 | 1.8 ± 0.1 | 59 ± 7.4 | 65 ± 4.6 |
Data are shown as mean ± SD. Unstabilized blood from haemophilia A (n = 3) or haemostatically normal dogs (n = 3) was spiked with rcFVIIa or rhFVIIa as indicated and run in duplicates in kaolin-activated thromboelastography (kaolin-TEG). rcFVIIa, recombinant canine factor VIIa; rhFVIIa, recombinant human FVIIa (NovoSeven®); R, reaction time; K, clotting time; α, angle; MA, maximum amplitude; –, no value.