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. 2014 Feb 12;13:41. doi: 10.1186/1475-2840-13-41

Figure 1.

Figure 1

Vaspin activates AMPK in HAECs. HAECS were incubated with 100 ng/mL vaspin for different amounts of times (A and B), or with different concentrations of vaspin or vehicle for 1 hr at 37°C (C and D). Phosphorylated AMPK and ACC were detected by Western blotting using phospho-specific antibodies for AMPK and ACC (top). Levels of phosphorylated AMPK and ACC were normalized against total levels of AMPK and ACC (bottom). Data are shown as mean values ± SEM of five independent experiments. 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) was used as a positive control in C and D. *p < 0.05 vs. untreated cells (Control), †p < 0.05 vs. cells treated with 25 ng/mL vaspin.