Figure 3.

Vaspin inhibits TNFα-induced expression of adhesion molecules. The effect of vaspin on TNFα-induced expression of ICAM-1 (A), VCAM-1 (A), E-selectin (B), and MCP-1 (B). The protein level and mRNA expression of each adhesion molecule was measured at 24 hr after treatment with 100 ng/mL vaspin in the presence or absence of 10 ng/mL TNFα. Data shown are representative Western blots (top panels) and mean values ± SEM of three independent experiments (bottom panels). The fold change in mRNA expression compared to levels in untreated cells is shown. ^*p < 0.05 vs. untreated cells, and ^†p < 0.05 vs. cells treated with TNFα alone.