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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Matrix Biol. 2013 Aug 15;33:47–53. doi: 10.1016/j.matbio.2013.08.003

Figure 1. Collagen V is a quantitatively minor component of tendon and comparably expressed in tenocyte cultures.

Figure 1

Collagens I and V were analyzed from P4 tendons (A–C) and cultured tenocytes (D, E). (A) Collagen I extracted from tendons was present as fully processed collagen. (B, C, E) Collagen V extracted from developing tendons and cultured tenocytes was present as the partially processed tissue form. Collagen V is present in significantly smaller amounts than collagen I. Based on band pixel density analysis of gels comparable to those in Panels A (anti-collagen I) and C (anti-collagen V), there is an ~80:1 ratio of extractable collagen I to collagen V in the developing tendon. (D, E) A similar minor quantitative contribution of collagen V was observed in the cell culture proteins. In tenocyte cultures collagen I was inefficiently processed with most of it present as unprocessed procollagen I or partially processed pC collagen I. Demarcations between panels indicate different exposures of a single gel to optimize signal for a specific protein. Separately bordered panels indicate different gels.