Fig. 3.

Drug treatments increase yeast lifespan. A. The CLS of sch9Δ cells (PF102) is enhanced by treating cells with myriocin (Myr). Data represent the mean ± SEM of survival (* p<0.05, ** p<0.01, No Myr vs 300 ng/ml Myr). Reproduced with permission [47]. B. Treatment of wild-type (DBY746) yeast cells with a low dose of myriocin plus rapamycin produces a synergistic enhancement in CLS. Cells were incubated with no drug, 45 ng/ml myriocin (Myr, 112 nM), 450 pg/ml rapamycin (Rap. 0.49 nM), 45 ng/ml Myr plus 450 pg/ml Rap. Data are for the mean ± SEM of viable cells in triplicate cultures. The dotted straight line with an arrowhead indicates an increase in the CLS of cells treated with both drugs that is greater than the additive effect on CLS of each drug treatment compared to untreated cells (additive effect is indicated by a dashed survival curve). The p value for the lifespan increase is computed using the area under the viability curves. Reproduced with permission [74]