Fig. 4.

Detection of CHS mutation in gamma-induced M3 populations of P. x hybrida MD. The numbers represent DNA pools from 25 M3 plants. The lack of amplification in pools 2, 4, 6, 14, 17 and 18 indicate mutated sequence. Plants from pool 2 were individually analyzed and the mutant CHS gene was sequenced; schematic representation of the mutation in the protein and deduced amino-acid sequence of the mutated allele are represented. Representative seed coat and vegetative anthocyanin pigmentation under monochromatic blue light are represented for wild type and mutant plants