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Steady state mRNA levels of the genes CHS, PH4 and CO in leaves of wild type and mutant examined by RT-PCR. WT: wild type Mitchell Diploid plants. chs, ph4_1, co_1: mutant plants. Positive reference control corresponds to transcripts of α-tubulin gene. A reaction mixture without template cDNA was used to confirm the absence of amplification from genomic DNA contamination of the RNA samples (RT-)
