Table 1.
Summary of the phenotypes of the petunia mutants identified by reverse genetics. The data correspond to in vivo analyses from the M3 isolated individual plants, grown in the greenhouse under 25 °C ± 5 °C, 12 h photoperiod provided by natural light and fluorescent supplementation (200 μmol.m−2.s−1). The numbers represent mean values ± standard errors from two independent evaluations and statistical significance is represented by Tukey test at P > 0.05
| Mutant | ||||
|---|---|---|---|---|
| Stage | chs1(Δ63) | ph4-1(D128→S) | co1 (ΔP348, R349) | Wild type |
| Vegetative | ||||
| Total chlorophyll (μg.mg−1) | 25.32 ± 2.24 | 25.56 ± 3.17 | 21.56 ± 3.01 | 25.41 ± 1.78 |
| Total anthocyanin (A535.mg−1) | 17.11 ± 3.25 | 18.02 ± 4.66 | 18.39 ± 4.49 | 18.54 ± 4.34 |
| Leaf pH | 6.20 ± 1.05 | 6.50 ± 0.94 | 6.20 ± 1.83 | 6.23 ± 0.93 |
| Leaf area (mm2) | 6.09 ± 2.91a | 6.21 ± 3.34a | 5.67 ± 3.76b | 5.98 ± 3.87ab |
| Internode length (cm) | 11.91 ± 3.11a | 11.82 ± 2.54a | 16.91 ± 3.23b | 11.85 ± 2.67a |
| Days to flowering | 18 ± 2.0 | 17 ± 1.7 | 25 ± 3.5 | 21 ± 2.2 |
| Reproductive | ||||
| Number of flowers per 30 days | 14 ± 1.3 | 14 ± 1.5 | 12 ± 1.8 | 14 ± 1.6 |
| Flower longevity (days) | 8.33 ± 1.12 | 7.00 ± 0.89 | 7.03 ± 1.76 | 7.55 ± 2.05 |
| Corolla pH | 5.70 ± 1.12a | 6.30 ± 0.87b | 5.60 ± 1.02a | 5.56 ± 0.99a |