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. 1978 May;75(5):2363–2367. doi: 10.1073/pnas.75.5.2363

Genetic analysis of the human cell surface: antigenic marker for the human X chromosome in human-mouse hybrids.

B P Dorman, N Shimizu, F H Ruddle
PMCID: PMC392553  PMID: 353811

Abstract

Somatic cell hybrids of human fibroblasts and mouse A9 cells, carrying only a portion of the human X chromosome in a mouse chromosome background, were injected into C3H mice. The resulting mouse anti-hybrid cell antisera contain antibodies found to be human specific and to react with only those hybrid cells carrying the human X chromosome, as confirmed by essentially perfect concordance between antibody binding assayed by indirect immunofluorescence and presence of the human X-linked enzyme hypoxanthine phosphoribosyl-transferase determined by autoradiographic assay of [3H] hypoxanthine utilization. Heterogeneous mixtures of hybrid cells may be analyzed into fluorescent (X plus) and nonfluorescent (X minus) subpopulations and fractionated viably by using a fluorescence-activated cell sorter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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