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Figure 1. Human colon cancer cell lines exhibit differential activation of MAPKs and expression levels of MKP1. (A) Western blot analysis of total cell extracts from WiDr, LoVo, CaCo2, and HCT116 cells using primary antibodies to phospho-JNKs (P-JNK), JNKs (JNK), phospho-ERKs (P-ERK), ERK1/2 (ERK), phospho-p38 (P-p38), p38 (p38), MKP1 (MKP1), and actin (Actin). (B) RT-PCR analysis of MKP1 mRNA expression normalized to that of GAPDH.
