Figure 1. BBMD3 inhibited cell viability in KHOS, G292, and MG-63 human osteosarcoma cells. (A) Structures of berbamine (BBM) and berbamine derivative 3 (BBMD3). (B) KHOS, G292, and MG-63 cells were treated with 0, 1, 3, 5, 8, and 10 µM BBMD3 for 24 h and 48 h and viability was determined by MTS assay as described in Methods. Each experiment was performed in triplicate. Each bar graph represents the mean, and the error bars represent ± SD.