Figure 3. Smad4 knockdown enhances CRC liver metastasis in vivo. (A) The morphology of metastatic liver in CT26-WT group, CT26-NC group, and Smad4KD group. Liver metastasis was examined 25 d after injecting CT26-WT, CT26-NC, and Smad4KD cells into the spleens of Balb/c mice. (B) The average weight of the metastatic liver in each group (n = 6). There was significant difference in metastatic liver weight between the CT26-NC group and the Smad4KD group (1.68 ± 0.52 g vs 3.22 ± 0.99 g). (C) The survival curve of mice in CT26-NC group and Smad4KD group (n = 10).