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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Invest Dermatol. 2013 Jul 26;134(2):405–414. doi: 10.1038/jid.2013.320

Figure 4. Matriptase and HAI-1 in an organotypic skin culture.

Figure 4

a. Expression and zymogen activation of matriptase. Primary human keratinocytes were grown in monolayer (M) or in an organotypic skin culture system (R). Cells were harvested on the day before they were lifted to the air-liquid interface and analyzed by immunoblot for total matriptase (Total MTP). The appearance of the 120-kDa matriptase complex with HAI-1 indicates the zymogen activation of matriptase. b and c. Expression of matriptase and HAI-1 over the course of epidermal differentiation. Organotypic skin cultures on Days 0, 3, 5, and 9 after lifting them to the air-liquid interface were analyzed by immunohistochemistry for total matriptase (Total MTP) using mAb M24 and HAI-1 (HAI-1) using mAb M19. Nuclei were counterstained blue with hematoxylin. Scale bar: 20 μm.